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在线固相萃取-高效液相色谱法(HPLC)测定煎炸用植物油中苯并(a)芘的含量 |
Determination of benzo(a)pyrene content in frying vegetable oils by on-line solid phase extraction-high performance liquid chromatography |
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DOI: |
中文关键词: 苯并(a)芘 在线固相萃取 HPLC 煎炸用植物油 |
英文关键词:benzo(a)pyrene on-line solid phase extraction HPLC frying vegetable oil |
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中文摘要: |
建立了在线固相萃取-高效液相色谱法(HPLC)测定煎炸用植物油中苯并(a)芘(BaP)含量的方法。样品用异丙醇-环己烷(体积比1∶1)溶解,经在线固相富集柱净化浓缩后,C18色谱柱分离,荧光检测器进行检测。结果表明:BaP在0.2~10 ng/mL质量浓度范围内与峰面积呈现良好的线性关系,相关系数0.999 9,方法的检出限为0.50 μg/kg;在BaP加标量为5~15 ng范围内,BaP加标回收率为101%~106%,RSD为2.6%~3.2%。该方法具有操作简单、毒性低、耗时短、重复性好和准确度高等优点,适用于大批量煎炸用植物油中BaP的检测。 |
英文摘要: |
A method for determining benzo(a)pyrene (BaP) content in frying vegetable oil by on-line solid phase extraction-high performance liquid chromatography was established. The samples were dissolved in isopropyl alcohol-cyclohexane(volume ratio 1∶1), cleaned up using on-line solid phase concentration column, and separated by C18column, then detected by fluorescence detector. The results showed that the BaP had good linear relationship in the range of 0.2-10 ng/mL, correlation coefficent was 0。999 9, and the limit of detection was 0.50 μg/kg. The recovery was 101%-106%, and RSD was 2.6%-3.2% with addition of 5-15 ng BaP in samples.This method had the items of simple operation, low toxicity, short time-consumption, good repeatability and accuracy, and could be applied in the detection of BaP in multitudinous frying vegetable oils. |
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