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| 解脂耶氏酵母β氧化基因敲除菌的构建及挥发性脂肪酸的利用 |
| Construction of Yarrowia lipolytica mutant with β-oxidation gene knockout and utilization of volatile fatty acids |
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| DOI： |
| 中文关键词: 解脂耶氏酵母 β氧化 长链脂肪酸 挥发性脂肪酸 |
| 英文关键词:Yarrowia lipolytica β-oxidation long-chain fatty acid volatile fatty acid |
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| 中文摘要: |
| 为了抑制解脂耶氏酵母长链脂肪酸的β氧化，使其稳定地积累，通过基因调控途径构建了4株脂肪酸β氧化基因敲除菌株，并研究了重组菌株利用挥发性脂肪酸作为碳源合成长链脂肪酸的能力。结果表明：分别敲除pox2、pox3和pex10的3株单基因敲除菌株及同时敲除pox2和pox3的组合基因敲除菌株构建成功，4株重组菌株均可以利用挥发性脂肪酸进行生长。与原始菌株相比，polf-Δpox2Δpox3和polf-Δpex10总脂产量（长链脂肪酸总和）在发酵后期并未因脂肪酸的β氧化而出现明显下降，是可以利用挥发性脂肪酸且可实现脂质积累的重组菌株。 |
| 英文摘要: |
| In order to inhibit the β-oxidation of long-chain fatty acids in Yarrowia lipolytica and accumulate fatty acids stably, four recombinant strains with β-oxidation gene knockout were constructed by gene regulatory pathway. The ability of recombinant strains accumulating large amount of long-chain fatty acids with volatile fatty acids as carbon source was studied. The results showed that pox2, pox3, pex10，pox2 and pox3 were successfully knocked out, and four recombinant strains could grow with volatile fatty acids. Compared with the original strain, the total yield of lipid （long-chain fatty acids） of polf-Δpox2Δpox3 and polf-Δpex10 did not decrease significantly by β-oxidation of long-chain fatty acids in the later stage of fermentation, so they could utilize volatile fatty acids and achieve lipid accumulation. |
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