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Construction of Yarrowia lipolytica mutant with β-oxidation gene knockout and utilization of volatile fatty acids
  
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KeyWord:Yarrowia lipolytica  β-oxidation  long-chain fatty acid  volatile fatty acid
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NI Lijuan，ZHANG Baixi，CHEN Wei，etc  
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Abstract:
      In order to inhibit the β-oxidation of long-chain fatty acids in Yarrowia lipolytica and accumulate fatty acids stably, four recombinant strains with β-oxidation gene knockout were constructed by gene regulatory pathway. The ability of recombinant strains accumulating large amount of long-chain fatty acids with volatile fatty acids as carbon source was studied. The results showed that pox2, pox3, pex10，pox2 and pox3 were successfully knocked out, and four recombinant strains could grow with volatile fatty acids. Compared with the original strain, the total yield of lipid （long-chain fatty acids） of polf-Δpox2Δpox3 and polf-Δpex10 did not decrease significantly by β-oxidation of long-chain fatty acids in the later stage of fermentation, so they could utilize volatile fatty acids and achieve lipid accumulation.
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