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| Yarrowia lipolytica H222（pox1-6缺陷）利用苹果渣酶解液与葡萄糖发酵合成微生物油脂的对比研究 |
| Comparison of oil production from enzymatic hydrolysate of apple pomace and glucose by Yarrowia lipolytica H222 （pox1-6-） |
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| DOI： |
| 中文关键词: 苹果渣酶解液 葡萄糖 微生物油脂 脂肪酸 |
| 英文关键词:enzymatic hydrolysate of apple pomace glucose microbial oil fatty acid |
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| 中文摘要: |
| 分别以苹果渣酶解液和葡萄糖作为发酵培养基的碳源，以Yarrowia lipolytica H222（pox1-6 缺陷）为实验菌株合成微生物油脂。分析比较发酵液中细胞干重、总油脂含量、总油脂产量及脂肪酸组成。结果表明：以苹果渣酶解液为碳源的发酵液中细胞干重、总油脂含量、总油脂产量、脂滴大小均较以葡萄糖为碳源的发酵液有显著增高；当发酵至3 d时，80 g/L总糖含量的苹果渣酶解液为碳源的发酵液中总油脂产量达到最高（0.088 mg/mL），明显高于以葡萄糖为碳源的发酵液中总油脂产量，并且不同碳源的发酵液中主要脂肪酸组成和含量也存在明显差异。因此，苹果渣酶解液更有利于Yarrowia lipolitica H222（pox1-6缺陷）产生油脂。 |
| 英文摘要: |
| Microbial oil was synthesized using enzymatic hydrolysate of apple pomace and glucose as carbon source of fermentation medium and Yarrowia lipolytica H222（pox1-6-）as experimental strain. The dry cell weight, total oil content, total oil productivity and fatty acid composition in fermentation broth were mainly compared and analyzed. The results showed that when the carbon source was enzymatic hydrolysate of apple pomace, the dry cell weight, total oil content, total oil productivity and oil droplet increased significantly. When fermentation time was 3 d, the total oil content was the highest（0.088 mg/mL） using enzymatic hydrolysate of apple pomace with 80 g/L of total sugar content as carbon source, which was far greater than that of glucose. And the fatty acid composition and content of fermentation broth had obvious differences using different carbon sources. Therefore, using enzymatic hydrolysate of apple pomace as carbon source was more beneficial for Yarrowia lipolytica H222（pox1-6-）to synthesize microbial oil. |
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