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油茶蒲中总三萜提取工艺研究
Extraction process of total triterpenoids from fruit hull of oil-tea camellia
  
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中文关键词:  油茶蒲  总三萜  浸提  正交试验
英文关键词:fruit hull of oil-tea camellia  total triterpenoids  extraction  orthogonal experiment
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SUN Qianhong  
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中文摘要:
      以油茶蒲为原料，利用甲醇作为浸提剂，研究了总三萜的提取工艺。采用单因素试验考察了甲醇体积分数、料液比、浸提温度和浸提时间对总三萜提取效果的影响，并通过正交试验分析确定了总三萜的最佳提取工艺条件。结果表明：影响总三萜提取的因素主次顺序为浸提时间＞料液比＞甲醇体积分数＞浸提温度；最佳提取工艺条件为甲醇体积分数80%、料液比1∶?25、浸提温度80?℃、浸提时间2.0 h，在此条件下，总三萜产率为0.115%。
英文摘要:
      With methanol as extraction agent, the extraction process of total triterpenoids from fruit hull of oil-tea camellia was studied. The effects of volume fraction of methanol, ratio of solid to liquid, extraction temperature and extraction time on extraction effect were studied by single factor experiment, and the extraction conditions were optimized by orthogonal experiment. The results showed that the extraction time had the greatest effect on extraction of total triterpenoids, followed by ratio of solid to liquid, volume fraction of methanol and extraction temperature. The optimal process conditions were obtained as follows: volume fraction of methanol 80%, ratio of solid to liquid 1∶?25, extraction temperature 80?℃ and extraction time 2.0 h. Under these conditions, the yield of total triterpenoids was 0.115%.
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