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| Ultrasound-assisted extraction of polysaccharides from Brassica juncea L. seed meal and its antioxidant activity |
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| DOI： |
| KeyWord:Brassica juncea L. seed meal polysaccharides ultrasound-assisted extraction antioxidant activity response surface methodology |
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| Abstract: |
| The ultrasound-assisted extraction of polysaccharides from Brassica juncea L. seed meal was optimized with extraction rate as index by response surface methodology based on single factor experiment. The antioxidant activities of the extracted polysaccharides were measured by scavenging DPPH· and ·OH. The results showed that the optimal extraction conditions were obtained as follows: extraction temperature 51?℃, extraction time 25 min, ultrasonic power 280 W, ratio of solid to liquid 1∶?40. Under these conditions, the extraction rate of polysaccharides was 14.18%. The half-scavenging rates （IC50） on DPPH· of the extracted polysaccharides and BHT were 0.177 mg/mL and 0.107 mg/mL, respectively. The IC50 on ·OH of the extracted polysaccharides and BHT were 0.24 mg/mL and 0.22 mg/mL, respectively. The polysaccharides from Brassica juncea L. seed meal exhibited powerful antioxidant activity. |
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