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| 两种复合诱变方法选育掷孢酵母高产油脂菌株 |
| Breeding of high oil yield strain of Sporobolomyces reseus by two compound mutagenesis methods |
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| DOI： |
| 中文关键词: 掷孢酵母 紫外-亚硝基胍复合诱变 紫外-硫酸二乙酯复合诱变 高产油脂菌株 |
| 英文关键词:Sporobolomyces reseus UV combined with NTG mutagenesis UV combined with DES mutagenesis high oil yield strain |
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| 中文摘要: |
| 以掷孢酵母（Sporobolomyces reseus）As.2.618为原始菌株，进行紫外-亚硝基胍和紫外-硫酸二乙酯两种复合诱变筛选，以获得高产油脂菌株。采用紫外-亚硝基胍复合诱变及马来酰肼初筛，突变株UV-NTG-2经摇瓶发酵复筛，生物量、油脂含量、油脂产量分别为39.01 g/L、19.83%、7.74 g/L，较原始菌株分别提高了42.42%、52.30%、116.81%。采用紫外-硫酸二乙酯复合诱变，经浅蓝菌素初筛及摇瓶复筛，突变株UV-DES-2的生物量、油脂含量、油脂产量分别为38.96 g/L、20.80%、8.10 g/L，较原始菌株分别提高了42.24%、59.75%、126.89%。诱变菌株的油脂脂肪酸组成中C18∶1的相对含量较原始菌株分别提高了11.88个百分点和6.69个百分点，且C20∶1的含量降低，说明突变菌株油脂的流动性较原始菌株有所提高。经5次连续传代表明，诱变菌株遗传性状稳定。 |
| 英文摘要: |
| Sporobolomyces reseus As.2.618 was screened by two compound mutagenesis methods UV combined with NTG and UV combined with DES to obtain high oil yield strain. UV combined with NTG added by maleic hydrazide was applied to obtain UV-NTG-2 strain，its biomass, oil content and oil yield reached 39.01 g/L, 19.83% and 7.74 g/L respectively, increasing by 42.42%, 52.30% and 11681% compared with the original strain. UV-DES-2 strain was mutated by UV combined with DES and screened by cerulenin.Its biomass, oil content and oil yield reached 38.96 g/L, 20.80% and 8.10 g/L respectively, increasing by 42.24%, 59.75% and 126.89% compared with the original strain. In addition, the relative contents of C18∶1 in the two mutant strains oils increased by 11.88 percentage points and 6.69 percentage points compared with the original strain, respectively. Furthermore, both of their relative contents of C20∶1 decreased, which indicated that the oil fluidity of the mutant strains improved. The mutant strains had stable genectic characters after five serial passages. |
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