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| Production of high protein and polypeptide rapeseed meal by microbial fermentation |
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| KeyWord:rapeseed meal crude protein polypeptide fermentation |
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| Abstract: |
| With rapeseed meal as raw material, the contents of crude protein and polypeptide as evaluation indexes, the strain combination and fermentation conditions were studied by single strain fermentation and multi-strains(Lactobacillus plantarum, Aspergillus niger, Bacillus subtilis) fermentation. The results showed that the contents of crude protein and polypeptide in rapeseed meal fermented by Aspergillus niger were the highest by single strain fermentation, reaching 38.4% and 3.682% respectively.The optimal inoculation amounts of Lactobacillus plantarum, Aspergillus niger, Bacillus subtilis were 4%, 6% and 6% respectively, and the optimal fermentation conditions were obtained as follows: ratio of water to material 1.7∶?1, fermentation temperature 34?℃ and fermentation time 48 h. Under these conditions, the contents of crude protein and polypeptide were 42.5% and 7.86% respectively. After fermentation, glucosinolate content reduced to 21.637 μmol/g, and the contents of phytic acid and tannin were 0.27% and 0.41 mg/g respectively. High protein, high polypeptide and low toxicity rapeseed meal feed was obtained. |
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