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Synthesis of phosphatidyl-fructose |
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DOI: |
KeyWord:phosphatidyl-fructose phospholipase D soybean lecithin |
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Abstract: |
Phosphatidyl-glycosides (PtdGlc) is composed of saturated fatty acyl chains(C18∶?0 and C20∶?0) and the polar head of the glycosides. Phosphatidyl-fructose belongs to one of the phosphatidyl-glycosides. With soybean lecithin and fructose as raw materials, phospholipase D as catalyst, an enzymatic method for synthesizing phosphatidyl-fructose in aqueous system was developed. Through the regulation of substrate ratio, pH, reaction temperature and reaction time, the optimal reaction conditions of phosphatidyl-fructose were established as follows: reaction temperature 40?℃, pH 6, reaction time 12 h, molar ratio of fructose to soybean lecithin 1∶?45, enzyme dosage 90 U/mL. Under the optimal reaction conditions, the synthesis rate of phosphatidyl-fructose in aqueous system was 90% or so. |
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