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| 磷脂酰果糖的合成方法研究 |
| Synthesis of phosphatidyl-fructose |
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| DOI： |
| 中文关键词: 磷脂酰果糖 磷脂酶D 大豆卵磷脂 |
| 英文关键词:phosphatidyl-fructose phospholipase D soybean lecithin |
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| 中文摘要: |
| 新型磷脂酰糖苷由饱和脂肪酰基链（C18∶0和C20∶0）以及糖苷极性头部组成，磷脂酰果糖属于磷脂酰糖苷中的一种。以大豆卵磷脂、果糖为原料，研究开发了水相体系中磷脂酶D催化酶法合成磷脂酰果糖的方法。通过对基质配比、pH、反应温度、反应时间等的调控，建立了磷脂酰果糖的最佳合成条件为：反应温度40?℃，pH 6，反应时间12 h，底物（果糖与大豆卵磷脂）摩尔比1∶45，酶添加量 90 U/mL。在最佳合成条件下，磷脂酰果糖合成率在90%左右。 |
| 英文摘要: |
| Phosphatidyl-glycosides （PtdGlc） is composed of saturated fatty acyl chains（C18∶?0 and C20∶?0） and the polar head of the glycosides. Phosphatidyl-fructose belongs to one of the phosphatidyl-glycosides. With soybean lecithin and fructose as raw materials, phospholipase D as catalyst, an enzymatic method for synthesizing phosphatidyl-fructose in aqueous system was developed. Through the regulation of substrate ratio, pH, reaction temperature and reaction time, the optimal reaction conditions of phosphatidyl-fructose were established as follows: reaction temperature 40?℃, pH 6, reaction time 12 h, molar ratio of fructose to soybean lecithin 1∶?45, enzyme dosage 90 U/mL. Under the optimal reaction conditions, the synthesis rate of phosphatidyl-fructose in aqueous system was 90% or so. |
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