Synthesis of phosphatidyl-fructose
  
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KeyWord:phosphatidyl-fructose  phospholipase D  soybean lecithin
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LIU Junyi, LIU Yanjun, LI Zhaojie, et al  
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Abstract:
      Phosphatidyl-glycosides (PtdGlc) is composed of saturated fatty acyl chains(C18∶?0 and C20∶?0) and the polar head of the glycosides. Phosphatidyl-fructose belongs to one of the phosphatidyl-glycosides. With soybean lecithin and fructose as raw materials, phospholipase D as catalyst, an enzymatic method for synthesizing phosphatidyl-fructose in aqueous system was developed. Through the regulation of substrate ratio, pH, reaction temperature and reaction time, the optimal reaction conditions of phosphatidyl-fructose were established as follows: reaction temperature 40?℃, pH 6, reaction time 12 h, molar ratio of fructose to soybean lecithin 1∶?45, enzyme dosage 90 U/mL. Under the optimal reaction conditions, the synthesis rate of phosphatidyl-fructose in aqueous system was 90% or so.
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