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超临界CO2法萃取树莓籽油的成分分析 |
Component analysis of raspberry seed oil extracted by supercritical CO2 |
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DOI: |
中文关键词: 超临界CO2萃取 树莓籽油 脂肪酸 甾醇 生育酚 多酚 |
英文关键词:supercritical CO2 extraction raspberry seed oil fatty acid sterol tocopherol polyphenol |
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中文摘要: |
采用超临界CO2技术萃取树莓籽油,利用GC法测定脂肪酸组成,GC-MS联用法测定甾醇组成,HPLC测定生育酚含量,紫外可见分光光度计测定多酚含量。结果表明:超临界CO2萃取树莓籽油的平均出油率为17.90%±0.3%;共检测到10种脂肪酸,其中亚油酸相对含量(57.44%)最高,其次是α-亚麻酸(26.58%)、油酸(11.94%)和棕榈酸(2.10%);脂肪伴随物中,共检测出7种甾醇,总甾醇含量为861.85 mg/100 g;γ-生育酚、α-生育酚和δ-生育酚含量分别达到799.248、602.996 mg/kg及13.371 mg/kg;多酚平均含量为(341.19±8.94)mgGAE/kg。此研究为树莓籽油及其他蔷薇科植物油的进一步开发利用奠定了基础。 |
英文摘要: |
The supercritical CO2was used to extract the raspberry seed oil, the fatty acid composition was determined by GC, the sterol composition was determined by GC-MS, the content of tocopherol was determined by HPLC,and the polyphenol content was determined by UV visible spectrophotometer. The results showed that the extraction of raspberry seed oil by supercritical CO2 was more efficient and simple, with an average oil yield of 17.90%±0.3%. A total of ten fatty acids were detected, of which the relative content of linoleic acid (57.44%) was the highest, followed by α-linolenic acid (26.58%), oleic acid (11.94%) and palmitic acid (2.10%). Seven kinds of sterols were detected, and the total content of sterols was 861.85 mg/100 g. In addition, the contents of γ-tocopherol, α-tocopherol and δ-tocopherol were 799.248, 602.996 mg/kg and 13.371 mg/kg, respectively. The average content of polyphenols was (341.19±8.94)mgGAE/kg. It laid the foundation for the further development and utilization of raspberry seed oil and other Rosaceae vegetable oils. |
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