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| Ultrasound-assisted enzymatic extraction and fatty acid composition of tea seed oil |
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| DOI： |
| KeyWord:ultrasound-assisted enzymatic extraction tea seed oil fatty acid |
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| Abstract: |
| With tea seed from huaihua area in hunan province as raw material, based on single factor experiment, the process of ultrasound-assisted enzymatic extraction of oil from tea seeds were optimized by orthogonal experiment, and the fatty acid composition of tea seed oil was analyzed by GC-MS. The results showed that the optimal process conditions of ultrasound-assisted enzymatic extraction of tea seed oil were obtained as follows: ratio of material to liquid 1∶?5, dosage of enzyme 0.6%, pH 5.8, ultrasonic hydrolysis temperature 55?℃, ultrasonic hydrolysis time 90 min and ultrasonic power 300 W. Under the optimal conditions, the yield of tea seed oil was (52.61±0.11%). 17 fatty acids were identified from the extracted tea seed oil by GC-MS, and unsaturated fatty acids, such as oleic acid(47.67%), linoleic acid(24.32%) and linolenic acid(4.26%) were the main fatty acids. |
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