|
| Rapid determination of four kinds of aflatoxins in peanut by UPLC-MS-MS |
| |
| DOI： |
| KeyWord:UPLC-MS-MS peanut aflatoxin rapid determination |
| FundProject: |
|
| Hits: 1314 |
| Download times: 461 |
| Abstract: |
| A UPLC-MS-MS method for detecting four kinds of aflatoxins in peanut was established. The samples were extracted in 80% acetonitrile-water solution by ultrasonic vibration, and purified using a multi-functional cleanup column. The sample was separated by Kinetex SB-C18 chromatographic column, subjected to gradient elution using methanol-0.1% formic acid solution as the mobile phase, then detected by triple-quadrupole mass spectrometry with multiple reaction monitoring （MRM）, and analyzed quantitatively by the external standard method. The results suggested that four kinds of aflatoxins presented good linearity within their respectively linear response ranges, and the coefficient of correlation were above 0.999; the methods limit of detection was 0.014-0.025 μg/kg; the recoveries of standard addition for blank samples were 72.25%-89.46% with the RSDs 4.01%-956%. Characterized by simple and quick pre-treatment, good purification effect, and low limit of detection, this method could be used for simultaneously rapid determination of various aflatoxins in peanut. |
| View full text View/Add Comment Download reader |
| Close |
|
|
|
