| The refining conditions of crude deep-sea fish oil were optimized, and the effects of H3PO4 volume fraction, excess alkali and activated clay dosage on the acid value, recovery rate and sensory quality of fish oil were investigated. The physicochemical indexes and fatty acid composition of refined fish oil were analyzed.The results showed that the optimal refining conditions were obtained as follows: degumming by adding 80% H3PO4 accounting for 1% of fish oil, deacidification by adding 4 mol/L NaOH and excess alkali 1.5% of fish oil, bleaching by adding activated clay accounting for 20% of fish oil. The physicochemical indicators of refined fish oil reached the first-grade standard of refined fish oil of aquatic industry（SC/T 3502—2000), and acid value decreased from (5.52±0.12) mgKOH/g to (0.29±0.11) mgKOH/g. The fatty acid composition didn’t change significantly during the refining. The content of DHA and EPA in the refined fish oil was about 38% of the total fatty acid content, and EPA and DHA accounted for 35.99% and 51.59% of the polyunsaturated fatty acid content respectively, so the quality of refined fish oil was excellent. |
