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| Extraction of total protein from Camellia oleifera kernel and two-dimensional electrophoresis analysis |
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| KeyWord:Camellia oleifera total kernel protein extraction method two-dimensional electrophoresis |
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| Abstract: |
| The total protein was extracted from Camellia oleifera kernel using different methods, and the conditions of two-dimensional electrophoresis were also explored and optimized to establish a set of optimal system of two-dimensional electrophoresis analysis for total protein in Camellia oleifera kernel. The results showed that total protein could be effectively extracted from Camellia oleifera kernel by the improved TCA-acetone method. 816 protein spots were obtained, and most of protein spots mainly concentrated at the isoelectric points pH 4.0-6.0. The optimal two-dimensional electrophoresis system was obtained as follows: length of IPG strip 17 cm with pH 4-7,isoelectric focusing program 250 V 0.5 h, 1 000 V 2 h, 8 000 V 5 h, 8 000 V 60 000 Vh and 500 V 24 h. Under these conditions, a 2-DE map with clear background and higher resolution could be obtained. |
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