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| 响应面优化水酶法提取裂壶藻油的工艺 |
| Optimization of aqueous enzymatic extraction of oil from Schizochytrium sp. by response surface methodology |
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| DOI： |
| 中文关键词: 裂壶藻油 响应面法 水酶法 清油得率 |
| 英文关键词:Schizochytrium sp. oil response surface methodology aqueous enzymatic method clear oil extraction rate |
| 基金项目: |
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| 中文摘要: |
| 以裂壶藻干藻粉为原料，以清油得率为评价指标，采用两步酶解法提取油脂。在单因素实验的基础上，对清油得率影响较大的碱性蛋白酶的作用条件应用响应面法进行优化，依据回归分析确定碱性蛋白酶的最适作用条件。结果表明，水酶法提取裂壶藻油的最适工艺条件为：料液比1∶?7，中性蛋白酶添加量7%，酶解温度45?℃，酶解时间3 h，酶解pH 6.5；碱性蛋白酶添加量10%，酶解温度68?℃，酶解时间6 h，酶解pH 9.4。在最适工艺条件下，裂壶藻清油得率可以达到（91.37±014）%。气相色谱-质谱分析裂壶藻油中不饱和脂肪酸含量为47.43%，其中DHA含量为35.09%。 |
| 英文摘要: |
| The oil was extracted from Schizochytrium sp. dry algae powder by two-step enzymatic method with clear oil extraction rate as evaluation indicator. On the basis of single factor experiment, using response surface methodology to optimize the conditions of alkaline protease that had a great influence on the clear oil extraction rate, the optimal alkaline protease conditions were determined by regression analysis. The results showed that the optimal extraction conditions were obtained as follows: solid-liquid ratio 1∶?7, neutral protease dosage 7%, enzymolysis temperature 45?℃, enzymolysis time 3 h, enzymolysis pH 6.5; alkaline protease dosage 10%, enzymolysis temperature 68?℃, enzymolysis time 6 h, enzymolysis pH 9.4. Under these conditions, the clear oil extraction rate of Schizochytrium sp. reached (91.37±0.14)%. GC-MS analysis results showed that the content of unsaturated fatty acids in Schizochytrium sp. oil was 47.43%, in which the DHA content was 35.09%. |
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