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| Substrate flow strategy for producing DHA by Schizochytrium sp.S31 and respiration characteristics analysis |
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| KeyWord:Schizochytrium sp.S31 substrate flow DHA CO2 release rate |
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| Abstract: |
| Considering that the mass concentrations of carbon and nitrogen in culture medium and their proportion had a significant effect on cell growth and oil accumulation, the effect of substrate flow strategy on production of DHA by Schizochytrium sp.S31 was studied using batch culture and intermittent flow method, and the correlation between CO2 release in fermentation tail gas and the accumulation of DHA was analyzed. The results showed that the optimal initial C/N ratio was 7∶1 by batch culture, and the final DHA content was 2.66 g/L under this condition. The optimal substrate flow strategy was that the yeast extract was intermittently fed in the first 80 h to make sure the mass concentration of yeast extract in culture medium reaching 5 g/L, and the glucose mass concentration was controlled at a constant level of 30 g/L, and the DHA content finally increased to 16.75 g/L.The respiration characteristics analysis results showed that there was a positive correlation between the average CO2 release rate and the accumulation of DHA after depletion of nitrogen. |
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