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Genome shuffling for rapid improvement of lipid content of Nannochloropsis oculata
  
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KeyWord:genome shuffling  Nannochloropsis oculata  lipid content
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LIU Hongquan, YUAN Sha, LU Yuxin,etc  
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Abstract:
      Nannochloropsis oculata as original strain was mutated by UV-light and EMS separately, and five mutant strains with high lipid content were selected. Two rounds of genome shuffling were carried out with the five mutant strains to mediate protoplasts fusion using PEG as fusogen. Finally, the Nannochloropsis oculata F2N2 and F2N4 were selected,and their lipid contents were 55.32% and 57.75%,respectively, higher than the original strain by 69.07% and 76.50%. Compared with the original strain in the same batch, the fatty acid composition of the Nannochloropsis oculata F2N2 lipid and F2N4 lipid didnt change a lot, but there was a big gap among fatty acid content.
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