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| 基因组改组技术快速提高拟微绿球藻油脂含量 |
| Genome shuffling for rapid improvement of lipid content of Nannochloropsis oculata |
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| DOI： |
| 中文关键词: 基因组改组 拟微绿球藻 油脂含量 |
| 英文关键词:genome shuffling Nannochloropsis oculata lipid content |
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| 中文摘要: |
| 以拟微绿球藻（Nannochloropsis oculata）为原始藻株，经过紫外线和甲基磺酸乙酯分别诱变处理，获得5株油脂含量有所提高的出发藻株。以PEG作为融合剂，对获得的5株出发藻株进行两轮递归式原生质体融合，筛选到遗传稳定的改组藻株F2N2和F2N4，其油脂含量分别达到55.32%与57.75%，较原始藻株分别提高了69.07%与76.50%。对拟微绿球藻的原始藻株和改组藻株的油脂脂肪酸组成及含量进行分析，结果表明改组前后拟微绿球藻的油脂脂肪酸组成变化不大，但脂肪酸含量有较大差别。 |
| 英文摘要: |
| Nannochloropsis oculata as original strain was mutated by UV-light and EMS separately, and five mutant strains with high lipid content were selected. Two rounds of genome shuffling were carried out with the five mutant strains to mediate protoplasts fusion using PEG as fusogen. Finally, the Nannochloropsis oculata F2N2 and F2N4 were selected,and their lipid contents were 55.32% and 57.75%,respectively, higher than the original strain by 69.07% and 76.50%. Compared with the original strain in the same batch, the fatty acid composition of the Nannochloropsis oculata F2N2 lipid and F2N4 lipid didnt change a lot, but there was a big gap among fatty acid content. |
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