Purification of phenolic compounds from Sacha Inchi shells by macroporous resin
  
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KeyWord:Sacha Inchi shell  phenolic compounds  macroporous resin  purification
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YANG Wan, GE Xuehui, ZHAO Chenwei,etc  
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Abstract:
      With Sacha Inchi(SI) shells as raw material, the crude phenolic compounds extract was extracted by 70% ethanol. The purification process conditions of phenolic compounds from SI shells by macroporous resins were studied, and six kinds of macroporous resins of AB-8,HPD300,NKA-9,D101,S-8 and DM130 were screened. The results showed that NKA-9 type resin was the most suitable resin for the purification of SI shell phenolic compounds. The optimal purification process conditions were obtained as follows: mass concentration of sample 10 mg/mL, flow rate of sample 2.0 mL/min, with 70% ethanol as eluent, elution rate 1.0 mL/min, and eluent volume 2.5 BV. Under the optimal conditions, the total phenol content in phenolic compounds from SI shells was 73.8%, which was 1.2 times higher than that in crude extract (33.2%).
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