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Extraction of high purity phosphatidylinositol
  
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KeyWord:soybean phospholipid powder  PI  freezing purification
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ZHU Meijin, CAO Dong, YIN Ru  
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Abstract:
      The extraction of high purity phosphatidylinositol (PI) was studied with alkaline ethanol as solvent and freezing purification using soybean phospholipid powder with low content of phosphatidylcholine (PC) as raw material. Based on single factor experiment, the optimal extraction conditions of PI were determined by response surface methodology as follows: freezing time 17 h, freezing temperature -20?℃, extraction temperature 40?℃, solid-liquid ratio 1∶?27, volume ratio of 98% ethanol to 25% ammonia 100∶?4, extraction time 40 min, extraction times three times. Under the optimal conditions, the content of PI in the product was 82.62%, and the yield was 14.29%.
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