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| Determination of ten kinds of antioxidants in edible oil by UPLC |
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| DOI： |
| KeyWord:UPLC edible oil antioxidant |
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| Abstract: |
| The method for simultaneous determination of ten kinds of antioxidants such as propyl gallate (PG), 2,4,5-trihydroxybenzoprostenone(THBP), tert-butylhydroquinone(TBHQ), isoamyl gallate (IAG), nordihydroguaiaretic acid (NDGA), tert-butyl p-hydroxyanisole (BHA), 2,6-di-tert-butyl-4-hydroxymethyl phenol (Lonox-100), octyl gallate (OG), 2,6-di-tert-butyl-p-methylphenol (BHT), and dicarboxylic acid dodecyl ester (DG) was established by UPLC. The antioxidants were extracted by acetonitrile with n-hexane saturation from the samples dissolved by n-hexane with acetonitrile saturation, and they were separated by C18column with 1% acetic acid solution and methanol as mobile phase for gradient elution. The ultraviolet detection wavelength was 280 nm and quantitative analysis was achieved by the external standard method. The results showed that the ten kinds of antioxidants had good linear relationship (r=0.998 7-0.999 6) and the low limit was less than 1.0 mg/kg. The recoveries and RSD were in the ranges of 89.31%-100.20% and 1.98%-5.78% respectively. The method was an accurate, reproducible and high sensitivity tool for simultaneous determination of various antioxidants in edible oil. |
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