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Relevance between thermodynamic characteristics, solubility, solution properties and surface hydrophobicity of soybean -conglycinin (7S) |
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DOI: |
KeyWord:β-conglycinin (7S) thermodynamic characteristics solubility relative molecular weight distribution hydrodynamic particle size ξ-potential surface hydrophobicity |
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Abstract: |
β-Conglycinin (7S) was prepared with soybean from six representative regions. Surface hydrophobicity of 7S was determined by ANS fluorescent probe method. Thermodynamic characteristics was analyzed by differential scanning calorimetry. Relative molecular weight distribution was determined by size exclusion gel chromatography. Hydrodynamic particle size distribution and ξ-potential were determined by Zeta Plus potential and laser particle size analyzer. The relationships between thermodynamic characteristics, solubility, solution properties and surface hydrophobicity of soybean 7S were studied. The results showed that there were significantly negative correlations between surface hydrophobicity of 7S and thermal denaturation temperature, denaturation enthalpy, and ξ-potential. There was extremely and significantly negative correlation between surface hydrophobicity and solubility of 7S. There was significantly positive correlation between surface hydrophobicity of 7S and average relative molecular weight and average particle size of soluble aggregates in 7S solution. |
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