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| 番茄红素纳米结构脂质体的制备 |
| Preparation of lycopene-loaded nanostructured lipid |
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| DOI： |
| 中文关键词: 番茄红素 纳米结构脂质载体 载量 水分散性 |
| 英文关键词:lycopene nanostructured lipid carrier loading amount water dispersibility |
| 基金项目: |
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| 中文摘要: |
| 对番茄红素纳米结构脂质体（lyco-NLC）的制备工艺进行了研究，以产品的平均粒径、多分散性系数（PDI）和包封率为指标，通过单因素实验和正交实验研究了物料因素和操作因素对lyco-NLC 的影响，确定最佳制备工艺。并对lyco-NLC进行了形貌观察和贮藏稳定性研究。结果表明，lyco-NLC的最佳制备工艺条件为：总脂质质量浓度7.5 g/100 mL，固液脂质量比3.5∶?1，表面活性剂吐温80质量浓度2 g/100 mL，均质压力50 MPa，均质次数3次。在最佳制备工艺条件下，lyco-NLC平均粒径为230 nm，PDI为0.23，包封率达到91.95%，产品中番茄红素载量高达4%，具有很好的水分散性。透射电子显微镜结果表明lyco-NLC粒子呈规则的球形，分散均匀。30 d的贮藏实验表明lyco-NLC具有良好的物理稳定性（平均粒径＜270 nm，PDI＜0.3）。 |
| 英文摘要: |
| The preparation process of lycopene-loaded nanostructured lipid (lyco-NLC) was studied. With product average particle size, polydispersity index(PDI) and encapsulation efficiency as indexes,the influences of material and operation factors on lyco-NLC were studied using single factor experiment and orthogonal experiment to obtain the optimal preparation process. The morphology of lyco-NLC was observed, and its storage stability was studied. The results showed that the optimal preparation process conditions were obtained as follows: total lipid mass concentration 7.5 g/100 mL, mass ratio of solid lipid to liquid lipid 3.5∶?1, surfactant Tween 80 mass concentration 2 g/100 mL, homogenization pressure 50 MPa, homogenization times 3. Under these conditions, the average particle size of lyco-NLC was 230 nm with PDI 0.23, the encapsulation efficiency was 91.95% and the lycopene loading amount in the product was up to 4%. The product possessed good water dispersibility. Particles of the lyco-NLC were found to be spherical and uniform in shape under transmission electron microscopy (TEM).The 30 d storage experiment results showed that lyco-NLC had good physical stability (average particle size <270 nm, PDI <0.3). |
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