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| Preparation of deep sea fish oil nanoemulsion |
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| KeyWord:deep sea fish oil nanoemulsion surfactant three phase diagram particle size |
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| Abstract: |
| The preparation method of deep sea fish oil nanoemulsion was investigated, and the physicochemical properties of the prepared nanoemulsion were measured. The deep sea fish oil was used as oil phase, and the proper emulsifier was screened from Span-20, Span-80, Tween-20 and Tween-80 by surfactant combination method. The results showed that with mixture of Span-80 and Tween-80 with volume ratio 19∶?81 as emulsifier and anhydrous ethanol as cosurfactant, when the volume ratio of mixed surfactant to deep sea fish oil and distilled water was 8∶?2∶?20, the prepared nanoemulsion was O/W type, apparent clear and transparent, the particle size ranged from 7 nm to 25 nm, and the polydispersity index (PDI) ranged from 0.15 to 0.25.The milk emulsion range of nanoemulsion was indicated by three phase diagram. The stability test results showed that the prepared nanoemulsion could keep stable after being centrifuged and unstable at high temperature, so it needed to be stored below 25?℃。 |
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