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| Determination of phospholipids in peanut by direct-injection HPLC-ELSD |
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| KeyWord:peanut phospholipids HPLC-ELSD quantitative analysis |
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| Abstract: |
| A method was established to determine phosphatidylcholine（PC），phosphatidyl ethanolamine（PE），phosphatidylinositol（PI）and phosphatidate（PA） in peanut by direct-injection HPLC-ELSD. A modified Folch method was used to extract the total fat and then the extract dissolved in the mobile phase was taken directly into HPLC. The HPLC method was performed on a column of L-Si (250 mm×4.6 mm, 5 μm) by gradient elution using hexane-isopropanol （volume ratio 2∶?3）and hexane-isopropanol- 25 mmol/L ammonium acetate（volume ratio 3.6∶?5.4∶?1）as the mobile phases and detected by evaporative light-scattering detector. The correlation coefficient of standard curve for mass concentration and peak area of each phospholipid in linearity range was above 0.995, as well as the average recovery rates of the four phospholipids in peanut were in the ranges of 93.59%-108.57% with the relative standard deviations(RSDs)less than 10%. The method had advantages of good reproducibility, high stability and simple pretreatment and it should be used for separation and determination of phospholipids. |
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