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Optimization of ultrasound-microwave-assisted extraction of proanthocyanidin from grape seeds by response surface methodology
  
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KeyWord:ultrasound-microwave  proanthocyanidin  grape seed  response surface methodology
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CHENG Haitao, SHEN Xianshuang  
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Abstract:
      With grape seeds produced in Hengshui as raw material, ultrasound-microwave was used to extract proanthocyanidin from grape seeds. The effects of ethanol volume fraction, ratio of liquid to material, ultrasonic power, ultrasonic time, microwave power and microwave time on proanthocyanidin yield were researched. Based on single factor experiment, the process of ultrasound-microwave-assisted extraction of proanthocyanidin from grape seeds was optimized by response surface methodology. The results showed that the optimal process conditions were obtained as follows: ethanol volume fraction 50%, ratio of liquid to material 21∶?1, ultrasonic power 400 W, ultrasonic time 32 min, microwave power 353 W, microwave time 3.2 min. Under the optimal conditions, the proanthocyanidin yield was 6.18%.
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