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| 产γ-亚麻酸诱变菌拉曼被孢霉HLY0902的发酵条件优化 |
| Optimization of fermentation conditions for gamma-linolenic acid production by mutant strain Mortierella ramanniana HLY0902 |
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| DOI： |
| 中文关键词: γ-亚麻酸 拉曼被孢霉 培养条件 培养基 |
| 英文关键词:gamma-linolenic acid Mortierella ramanniana culture condition culture medium |
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| 中文摘要: |
| 采用正交实验和单因素实验，分别对诱变菌拉曼被孢霉HLY0902的培养基及培养条件进行优化，以期提高γ-亚麻酸(GLA)的产量。结果表明：当发酵培养基组成为葡萄糖100 g/L、酵母浸粉10 g/L、KH2PO4 4 g/L、NaNO3 1 g/L、MgSO4·7H2O 0.5 g/L时，GLA的产量最大，可达1.05 g/L，较优化前提高了43.8%; 最优培养条件为接种量10%，装液量20%，发酵时间168 h, 适合菌体生长和油脂积累的pH为5.5、发酵温度为22?℃，适合GLA积累的pH为7.5、发酵温度为20?℃。通过该系列的优化研究，诱变菌拉曼被孢霉HLY0902产GLA的能力显著提高。 |
| 英文摘要: |
| The culture medium and culture conditions of the mutant strain Mortierella ramanniana HLY0902 were optimized by orthogonal experiment and single factor experiment in order to increase the production of gamma-linolenic acid(GLA). The results showed that the yield of GLA was up to 1.05 g/L in the medium containing glucose 100 g/L, yeast extract powder 10 g/L, KH2PO4 4 g/L, NaNO3 1 g/L, MgSO4 ·7H2O 0.5 g/L and increased by 43.8% compared with that before optimization. The optimal culture conditions were identified as follows: inoculation amount 10%, loading amount 20%, batch time 168 h. Furthermore, the optimal conditions for mycelium growth and oil accumulation were pH 5.5 and 22℃, and the optimal conditions for GLA accumulation were pH 7.5 and 20?℃. The results showed that the capacity of GLA production by mutant strain Mortierella ramanniana HLY0902 improved through the optimization. |
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