| Flavonoids were extracted from peony seed shell by ultrasound extraction method, and the effects of volume fraction of ethanol, solid-liquid ratio, ultrasonic power, extraction time and extraction temperature on the yield of total flavonoids were studied by single factor experiment. Based on this, the extraction conditions were optimized by response surface methodology. Using VC as control, the antioxidant activity of total flavonoids from peony seed shell was assessed by DPPH method. The results demonstrated that the optimal extraction conditions were obtained as follows: volume fraction of ethanol 60%, solid-liquid ratio 1∶?50, ultrasonic power 250 W, extraction time 50 min and extraction temperature 40?℃. Under these conditions, the yield of flavonoids was 13.66%. The scavenging ability of flavonoids extracted from peony seed shell on DPPH free radical was stronger than that of VC, and the antioxidant ability and mass concentration were in a concentration-dependent manner. |
