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高效液相色谱-电喷雾串联质谱法测定花生油中黄曲霉毒素B1的含量
Determination of AFB1 content in peanut oil by HPLC-ESI MS/MS
  
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中文关键词:  高效液相色谱-电喷雾串联质谱  黄曲霉毒素B1  花生油
英文关键词:HPLC-ESI MS/MS  AFB1  peanut oil
基金项目:
Author NameAffiliation
BAO Huimei  
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中文摘要:
      建立了高效液相色谱-电喷雾串联质谱检测花生油中黄曲霉毒素B1含量的方法，并探讨了最佳检测条件。结果表明：最佳检测条件为离子源温度110℃，毛细管电压3 500 V，电喷雾离子源（ESI+），鞘气压力270 kPa，辅助气压力104 kPa，磁接气流速0.1 mL/min，提取溶剂为甲醇。在最佳检测条件下，黄曲霉毒素B1在1.00~11.00 ng/mL范围内呈良好的线性关系，检出限为0.3 μg/kg，平均回收率为99.7%，RSD为0.27%。该测定方法具有简单、快速、灵敏度高、准确的优点，电喷雾质谱提供的离子对扫描能够很大程度上减少基质的干扰。
英文摘要:
      A method for the determination of AFB1 content in peanut oil was developed by HPLC-ESI MS/MS, and the detection conditions were optimized. The results showed that the optimal detection conditions were obtained as follows: ion source temperature 110?℃, capillary voltage 3 500 V, electrospray ionization (ESI+)， sheath gas pressure 270 kPa, auxiliary gas pressure 104 kPa, magnetic gas flow rate 0.1 mL/min, with methanol as extraction solvent. Under these conditions, AFB1 had good linear relationship in the range of 1.00-11.00 ng/mL, and the limit of detection, average recovery rate and RSD were 0.3 μg/kg, 99.7% and 0.27% respectively. The method had the items of simpleness, rapidness, sensitivity and accuracy, and the ion pair scanning provided by ESI-MS could reduce the matrix interference to a great extent.
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