| The optimal ultrasound-assisted extraction process of seed oil from Mentha haplocalyx was obtained through single factor experiment and orthogonal experiment with n-hexane as extraction solvent, then the fatty acid composition, physicochemical properties and acute toxicity of Mentha haplocalyx seed oil were analyzed. The results showed that the optimal extraction process of seed oil from Mentha haplocalyx was obtained as follows: ratio of material to liquid 1∶?10, ultrasonic time 50 min, ultrasonic power 300 W and ultrasonic temperature 50?℃. Under these conditions, the yield of seed oil could reach 25.6%. GC-MS analysis revealed that there were four kinds of fatty acids in Mentha haplocalyx seed oil, which were palmitic acid (544%), oleic acid (13.83%), linoleic acid (23.85%) and linolenic acid (56.88%). The unsaturated fatty acids accounted for 94.56%, which was about 17.4 times of saturated fatty acid content. The density, refractive index(n20D), iodine value, acid value and saponification value of the seed oil were 0.908 g/mL, 1.511, 148.6 gI/100 g, 087 mgKOH/g and 182.7 mgKOH/g, respectively. Mentha haplocalyx seed oil showed a significant scavenging effect on DPPH radical, and the scavenging rate increased with the rising of oil volume fraction while the increase degree decreased gradually. Acute toxicological test found that the mice did not show any behavior, signs, organ abnormalities and death during the experimental period. Mentha haplocalyx seed oil was a good vegetable oil resource with high content of unsaturated fatty acids and great development potential, and it did not exhibit acute toxicity. |
