| The protein of cactus fruit seed was extracted by the method of alkali extraction and acid precipitation. The effects of solid-liquid ratio, pH, extraction time and extraction temperature on the extraction rate of protein were studied by single factor experiment, and the optimal extraction conditions were determined. In addition, the protein functional properties such as water and oil absorption properties, foaming ability and foam stability, emulsifying ability and emulsion stability were analyzed, and the pH, NaCl concentration and protein mass concentration influencing the functional properties were discussed. The results showed that the optimal extraction conditions of alkali extraction and acid precipitation were obtained as follows: solid-liquid ratio 1∶?20, pH 10, extraction time 40 min and extraction temperature 40?℃. Under the optimal conditions, the extraction rate of protein was (68.17±0.33）%. The water and oil absorption properties were good, and the influences of pH, NaCl concentration and protein mass concentration on foaming ability, foam stability, emulsifying ability and emulsion stability were remarkable. |
