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Enzymatic fractionation of conjugated linoleic acid isomers to get functional monomers
  
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KeyWord:conjugated linoleic acid  Lipase AYS  isomer fractionation  recovery rate
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LIU Zheng, CHEN Huayong, WANG Yonghua，etc  
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Abstract:
      The two functional monomers of conjugated linoleic acid (CLA) was fractionated by Lipase AYS-catalyzed esterification of methanol and CLA.The process of esterification was optimized by single factor experiment as follows：Lipase AYS amount 180 U/g， reaction temperature 40?℃， buffer pH 6.5，molar ratio of CLA to methanol 1∶?1.After reaction for 8 h under the optimal conditions, the total esterification rate was 495%. After separation of methyl ester and fatty acid from reaction mixture，the fractionation efficiency of c9，t11-CLA in methyl ester phase and t10，c12-CLA in fatty acid phase were 862% and 80.0%，and the recovery rates of the two monomers were 72.4% and 54.2%, respectively.
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