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| 酶法拆分制备共轭亚油酸功能单体 |
| Enzymatic fractionation of conjugated linoleic acid isomers to get functional monomers |
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| DOI： |
| 中文关键词: 共轭亚油酸 Lipase AYS 异构体分离 回收率 |
| 英文关键词:conjugated linoleic acid Lipase AYS isomer fractionation recovery rate |
| 基金项目: |
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| 中文摘要: |
| 研究利用Lipase AYS催化甲醇和共轭亚油酸(CLA)进行酯化反应，以期对共轭亚油酸异构体进行拆分制备共轭亚油酸功能单体。通过考察酯化反应的影响因素，确定了最优反应条件为：Lipase AYS加酶量180 U/g，反应温度40?℃，CLA与甲醇摩尔比1∶?1，缓冲液pH 6.5。在最优条件下反应8 h，总酯化率为49.5％，分离产物可以得到甲酯相与脂肪酸相，甲酯相中c9，t11-CLA甲酯的拆分效率达到86.2%，脂肪酸相中t10，c12-CLA的拆分效率达到80.0%，两者回收率分别为72.4%和54.2%。 |
| 英文摘要: |
| The two functional monomers of conjugated linoleic acid (CLA) was fractionated by Lipase AYS-catalyzed esterification of methanol and CLA.The process of esterification was optimized by single factor experiment as follows：Lipase AYS amount 180 U/g， reaction temperature 40?℃， buffer pH 6.5，molar ratio of CLA to methanol 1∶?1.After reaction for 8 h under the optimal conditions, the total esterification rate was 495%. After separation of methyl ester and fatty acid from reaction mixture，the fractionation efficiency of c9，t11-CLA in methyl ester phase and t10，c12-CLA in fatty acid phase were 862% and 80.0%，and the recovery rates of the two monomers were 72.4% and 54.2%, respectively. |
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