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| 免疫亲和柱净化-高效液相色谱法测定玉米油中F-2毒素含量的研究 |
| Determination of F-2 toxin in corn oil by immune-affinity column purification and HPLC |
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| DOI： |
| 中文关键词: F-2毒素 玉米油 免疫亲和柱 高效液相色谱法 |
| 英文关键词:F-2 toxin corn oil immune-affinity column HPLC |
| 基金项目: |
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| 中文摘要: |
| 建立免疫亲和柱净化-高效液相色谱法测定玉米油中F-2毒素的方法。样品经前处理后通过免疫亲和柱净化，采用高效液相色谱仪-荧光检测器进行检测。该方法最佳测定条件为：选用IAC-AZ型免疫亲和柱，柱吸附容量4 μg；流动相乙腈-甲醇-水（体积比46∶?46∶?8），流速1.0 mL/min；提取溶剂乙腈-水（体积比90∶?10）。F-2毒素的质量浓度在0~250 ng/mL的范围内与峰面积呈良好的线性关系，方法检出限为0.06 μg/kg，平均回收率为92.46%，RSD为0.942%~1758%。该方法操作简便快捷、准确度高、重现性好，适用于玉米油中F-2毒素的测定。 |
| 英文摘要: |
| A method for determination of F-2 toxin in corn oil by immune-affinity column purification and HPLC was established. The sample was firstly pretreated, then purified by immune-affinity column and determined by HPLC with fluorescence detector. The optimal determination conditions were obtained as follows: IAC-AZ immune-affinity column, column adsorption capacity 4 μg, acetonitrile-methanol-water (volume ratio 46∶?46∶?8) as mobile phase, flow rate 1.0 mL/min, extraction solvent acetonitrile-water (volume ratio 90∶?10). The results showed that the mass concentration of F-2 toxin had good linear relation ship with peak area in the range of 0-250 ng/mL,the recovery was 92.46% with RSD 0942%-1.758%,and the limit of detection was 0.06 μg/kg. The method was simple and easy to operate with high accuracy and good reproducibility, and it was suitable for the determination of F-2 toxin in corn oil. |
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