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Determination of F-2 toxin in corn oil by immune-affinity column purification and HPLC
  
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KeyWord:F-2 toxin  corn oil  immune-affinity column  HPLC
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LUO Jianguang  
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Abstract:
      A method for determination of F-2 toxin in corn oil by immune-affinity column purification and HPLC was established. The sample was firstly pretreated, then purified by immune-affinity column and determined by HPLC with fluorescence detector. The optimal determination conditions were obtained as follows: IAC-AZ immune-affinity column, column adsorption capacity 4 μg, acetonitrile-methanol-water (volume ratio 46∶?46∶?8) as mobile phase, flow rate 1.0 mL/min, extraction solvent acetonitrile-water (volume ratio 90∶?10). The results showed that the mass concentration of F-2 toxin had good linear relation ship with peak area in the range of 0-250 ng/mL,the recovery was 92.46% with RSD 0942%-1.758%,and the limit of detection was 0.06 μg/kg. The method was simple and easy to operate with high accuracy and good reproducibility, and it was suitable for the determination of F-2 toxin in corn oil.
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