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| 拟微绿球藻中二十碳五烯酸提取工艺的比较研究 |
| Comparison of extraction process of eicosapentaenoic acid from Nannochloropsis oceanica |
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| DOI： |
| 中文关键词: 拟微绿球藻 超临界CO2萃取 溶剂提取 响应面法 EPA |
| 英文关键词:Nannochloropsis oceanica supercritical CO2 extraction solvent extraction response surface methodology EPA |
| 基金项目: |
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| 中文摘要: |
| 以拟微绿球藻为原料，比较不同有机溶剂体系及超临界CO2（SC-CO2）萃取对微藻油得率和EPA含量的影响，并采用响应面法对SC-CO2萃取工艺进行优化。结果表明：有机溶剂体系石油醚-丙酮-乙醇（体积比2∶?1∶?0.5）可以获得较好的萃取效果，此条件下微藻油得率为23.74%，EPA含量为31.34%；SC-CO2萃取最佳夹带剂为甲醇，最佳萃取工艺参数为萃取压力26 MPa、萃取温度48?℃、萃取时间168 min，此条件下微藻油得率为8.34%，EPA含量为25.66%。GC-MS分析显示，两种方法所得微藻油脂肪酸组成一致，且均以不饱和脂肪酸为主。考虑到设备成本、能耗等问题，溶剂浸提法相比于SC-CO2更具有一定优势。 |
| 英文摘要: |
| The effects of different organic solvent systems and supercritical CO2（SC-CO2）extraction on the yield and EPA content of microalgae oil were studied with Nannochloropsis Oceanica as the material. Response surface methodology was employed to optimize the SC-CO2 extraction conditions. The results showed that the optimal extraction process for solvent extraction was petroleum ether-acetone-ethanol (volume ratio 2∶?1∶?0.5). Under this condition, the yield of microalgae oil was 23.74%, and the EPA content accounted for 31.34%. The best co-solvent for SC-CO2 extraction was methanol, and the optimal extraction conditions were obtained as follows: extraction pressure 26 MPa, extraction temperature 48?℃ and extraction time 168 min. Under these conditions, the yield of microalgae oil was 8.34%, and EPA content reached 25.66%. GC-MS analysis results showed that the fatty acid compositions of microalgae oils extracted by two methods had no significant difference, mainly composed of polyunsaturated fatty acids. Considering the equipment cost, energy consumption and other issues, solvent extraction method had more advantage than SC-CO2. |
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