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| Reuse of liquid supernatant in the fermentation broth of allotrophic Chlorella vulgaris |
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| KeyWord:Chlorella vulgaris supernatant in the fermentation broth reuse formulation biomass |
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| Abstract: |
| The experiment of chlorella vulgaris growing with supernatant in the fermentation broth was carried out. Taking the biomass of chlorella vulgaris as the indexes, through the supernatant recycle ratio experiment, single factor experiment and L9(34) orthogonal experiment to optimize formulation, the feasibility to grow the chlorella vulgaris with supernatant was investigated. The results showed that the recycle ratio of supernatant accounted for 30% of the total fermentation broth with the best treatment effect. The optimal formulation was 2 g/L yeast powder, 0.3 g/L K2HPO4, 0.2 g/L MgSO4 and 0.5 mL microelement mother liquor. Under these technological conditions, the biomass of chlorella vulgaris in the end stage of fermentation in the first time recycling of supernatant was about 71% higher than control group. The second and third time recycling were higher than control group even decrease in turn. It proved that the chlorella vulgaris could promote its growth with the optimal formulation supernatant. The recycling of supernatan in the fermentation broth could save 30% of water consumption, 40% of the amount of nutrients and reduce environmental pollution. |
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