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| 异养小球藻发酵液上清液回用的试验研究 |
| Reuse of liquid supernatant in the fermentation broth of allotrophic Chlorella vulgaris |
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| DOI： |
| 中文关键词: 小球藻 发酵液上清液 回用 配方 生物量 |
| 英文关键词:Chlorella vulgaris supernatant in the fermentation broth reuse formulation biomass |
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| 中文摘要: |
| 采用发酵液上清液进行小球藻培养试验研究。以小球藻的生物量为指标，通过上清液回用比例试验、单因素试验和L9(34)正交试验进行配方优化，考察利用上清液培养小球藻的可行性。结果表明：上清液回用比例为30%（占配料用水量）处理效果最佳，在此基础上的配方优化结果为酵母粉2 g/L、K2HPO4 0.3 g/L、MgSO4 0.2 g/L、微量元素母液0.5 mL；在此工艺条件下，上清液首次回用小球藻在发酵末期的生物量比未添加上清液对照组高71%左右，二次、三次回用依次降低，但仍高于对照组，进一步验证了小球藻在优化配方下能够有效利用小球藻发酵液上清液促进自身生长，并且通过发酵液上清液的循环使用，可以节省30%的用水量、40%的营养物质(不含糖)用量并且可以减少环境污染。 |
| 英文摘要: |
| The experiment of chlorella vulgaris growing with supernatant in the fermentation broth was carried out. Taking the biomass of chlorella vulgaris as the indexes, through the supernatant recycle ratio experiment, single factor experiment and L9(34) orthogonal experiment to optimize formulation, the feasibility to grow the chlorella vulgaris with supernatant was investigated. The results showed that the recycle ratio of supernatant accounted for 30% of the total fermentation broth with the best treatment effect. The optimal formulation was 2 g/L yeast powder, 0.3 g/L K2HPO4, 0.2 g/L MgSO4 and 0.5 mL microelement mother liquor. Under these technological conditions, the biomass of chlorella vulgaris in the end stage of fermentation in the first time recycling of supernatant was about 71% higher than control group. The second and third time recycling were higher than control group even decrease in turn. It proved that the chlorella vulgaris could promote its growth with the optimal formulation supernatant. The recycling of supernatan in the fermentation broth could save 30% of water consumption, 40% of the amount of nutrients and reduce environmental pollution. |
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