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Screening of UV-mutated Chlorella zofingensis with high lipid content using flow cytometry
  
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KeyWord:Chlorella zofingensis  UV-mutated mutation  flow cytometry screening  high lipid content
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LI Qing, WU Hong, CAI Zhongzhen, etc  
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Abstract:
      In order to screen strain with high lipid content from Chlorella zofingensis, firstly Chlorella zofingensis was mutated by ultraviolet ray(UV), and then the cells of mutated Chlorella zofingensis were screened by flow cytometry after Nile red staining. The results showed that after being mutated by UV for 40 min and screened by flow cytometry, 90% of the mutated strains contained more lipid than wild control strain. The total lipid yield of the screened strain S16 reached 2.4 g/L, increasing by 50% compared with wild control strain.
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