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| 响应面法优化提取文冠果壳中多酚类物质及其体外抗氧化能力分析 |
| Response surface methodology optimizing extraction and in vitro antioxidant activity of Xanthoceras sorbifolia polyphenols |
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| DOI： |
| 中文关键词: 文冠果壳 多酚 体外抗氧化 超声波 |
| 英文关键词:Xanthoceras sorbifolia seed coat polyphenols in vitro antioxidant activity ultrasound |
| 基金项目: |
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| 中文摘要: |
| 采用超声波辅助提取文冠果壳多酚，以多酚得率为指标，在单因素实验基础上，采用Box-Behnken中心组合实验优化文冠果壳多酚的提取条件；以DPPH自由基清除能力、ABTS+自由基清除能力、铁还原能力为指标，考察文冠果壳多酚提取物的体外抗氧化能力。结果表明：超声波辅助提取文冠果壳多酚的最佳条件为料液比1∶?20、超声时间26.4 min、提取温度47?℃、超声波功率250 W，在最佳条件下文冠果壳多酚得率为5.64%；文冠果壳多酚提取物有较强的体外抗氧化能力，其清除DPPH自由基的IC50值为13.36 μg/mL，清除ABTS+自由基的IC50值为23.7 μg/mL，铁还原能力为 6.4 mg FeSO4/mg DW，可开发为天然抗氧化剂。 |
| 英文摘要: |
| In order to optimize the ultrasound-assisted extraction process of Xanthoceras sorbifolia polyphenols, based on single factor experiment, Box-Behnken experimental design and response surface methodology were employed with polyphenols yield as response value. The in vitro antioxidant activity of Xanthoceras sorbifolia polyphenols (XSSC) was evaluated by DPPH free radical scavenging capacity, ABTS+ free radical scavenging capacity and ferric reducing ability. The results showed that the optimal extraction conditions were determined as follows: material-to-liquid ratio 1∶?20, ultrasonic time 26.4 min, extraction temperature 47?℃, ultrasonic power 250 W. Under these conditions, the polyphenols yield was 5.64%. XSSC had excellent in vitro antioxidant activity, the IC50values of DPPH free radical scavenging capacity and ABTS+ free radical scavenging capacity of XSSC were 13.36, 23.7 μg/mL respectively, and the ferric reducing ability was 6.4 mg/mg. It had the potential to be explored as a nature antioxidant. |
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