| In order to optimize the ultrasound-assisted extraction process of Xanthoceras sorbifolia polyphenols, based on single factor experiment, Box-Behnken experimental design and response surface methodology were employed with polyphenols yield as response value. The in vitro antioxidant activity of Xanthoceras sorbifolia polyphenols (XSSC) was evaluated by DPPH free radical scavenging capacity, ABTS+ free radical scavenging capacity and ferric reducing ability. The results showed that the optimal extraction conditions were determined as follows: material-to-liquid ratio 1∶?20, ultrasonic time 26.4 min, extraction temperature 47?℃, ultrasonic power 250 W. Under these conditions, the polyphenols yield was 5.64%. XSSC had excellent in vitro antioxidant activity, the IC50values of DPPH free radical scavenging capacity and ABTS+ free radical scavenging capacity of XSSC were 13.36, 23.7 μg/mL respectively, and the ferric reducing ability was 6.4 mg/mg. It had the potential to be explored as a nature antioxidant. |
