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Enrichment, separation and purification of squalene from plant asphalt
  
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KeyWord:plant asphalt  squalene  centrifugation  molecular distillation  acticarbon  silica gel  adsorption
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XIAO Xiang，WANG Jialei，ZHANG Sheng，etc  
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Abstract:
      The enrichment process, separation and purification process of squalene from plant asphalt were studied. The squalene was enriched in plant asphalt by cold separation centrifugation and molecular distillation, then the effects of solvent polarity, adsorbent dosage and adsorption time on the content and yield of squalene were investigated. Based on single factor experiment, the separation and purification conditions were optimized by orthogonal experiment as follows: with acetone-petroleum ether (volume ratio 30∶?70) as solvent, mass ratio of silica gel to sample 1∶?1, mass ratio of acticarbon to sample 3∶?1. Under the optimal conditions, the content and yield of squalene were 87.34% and 88.69%,respectively.
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