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| Simultaneous determination of tocopherol, sesamin and sesamolin in sesame oil by normal phase-high performance liquid chromatography |
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| KeyWord:sesame oil tocopherol sesamin sesamolin normal phase-high performance liquid chromatography |
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| Abstract: |
| A method was developed for simultaneous determination of tocopherol, sesamin and sesamolin in sesame oil by normal phase-high performance liquid chromatography. The sample was dissolved by n-heptane, and eluted by tetrahydrofuran-n-heptane in a DIOL column, and detected by fluorescence detector. The results showed that sesamin and sesamolin presented good fluorescent characteristics and the analysis was carried out within 20 min. The evaluation of methodology indicated that tocopherol showed a good linearity in the range of 1.0-5.0 關g/mL while sesamin and sesamolin of 0.1-5.0 關g/mL with correlation coefficients (R2) above 0.99. The limits of detections (LODs) and the limits of quantifications (LOQs) of tocopherol, sesamin and sesamolin were 0.29-0.74 mg/kg and 0.91-2.10 mg/kg, respectively. The average recoveries of six analytes were 83.47%-104.57%, and the relative standard deviations (RSDs) were 0.38%-6.55%. Twelve samples including fragrant sesame oil, cold pressed sesame oil and extracted sesame oil were analyzed by this method. The results showed that main tocopherol in the sesame oil was 款 type and the contents of sesamin and sesamolin were high. The method could be used to simultaneously determine tocopherol, sesamin and sesamolin due to its simple operation, high sensitivity and good reproduction. |
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