| According to the principle of zearalenone(ZEN)dissolving in ethanol easily, the corn germ meal was washed by ethanol to remove ZEN, improve protein content and sensory quality. With corn germ meal as raw material, the effects of volume fraction of ethanol, ethanol washing temperature, ratio of material to liquid, ethanol washing time and ethanol washing times on the removal effect of ZEN were studied. Based on single factor experiment, the optimal removal conditions of ZEN from corn germ meal by ethanol washing method were obtained by orthogonal experiment as follows:volume fraction of ethanol 80%, ethanol washing temperature 40?℃, ratio of material to liquid 1∶?15, ethanol washing time 50 min and ethanol washing once. Under these conditions, the content of ZEN in the corn germ meal after ethanol washing decreased from 906.60 μg/kg to 179.21 μg/kg, which was less than 500 μg/kg required by national standard to forage, and the removal rate of ZEN reached above 80%. In addition, the protein content increased from 17.3% to 21.8%, and the sensory qualities such as color and flavor were improved. |
