Optimization of cultivation conditions of Monoraphidium dybowskii C29 in domestic sewage
  
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KeyWord:domestic sewage  Monoraphidium dybowskii C29  optimization culture  biomass  oil accumulation  sewage purification
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WANG Meng, LIU Pinghuai  
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Abstract:
      In order to obtain the optimal nutritional conditions of Monoraphidium dybowskii C29 growth in domestic sewage, and study how Monoraphidium dybowskii C29 took an effect on sewage purification, the experiment was performed by adding different dosages of BG11 medium, adjusting different initial pH and adding different mass concentrations of glucose into sewage to test the biomass, oil production, and nitrogen and phosphorus removal rates. The results showed that under the conditions of dosage of BG11 medium 50% in sewage, pH 7 and final mass concentration of glucose 0.5 g/L, the growth of Monoraphidium dybowskii C29 was the best, and the biomass and oil production were 2.74 g/L and 1.04 g/L respectively, at the same time, the removal rates of nitrogen and phosphorus in sewage were 86.09% and 96.64% respectively. Therefore, using domestic sewage to cultivate Monoraphidium dybowskii C29 could simultaneously gain the oil-producing microalgae culture and purification of sewage.
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