| With self-screened microalgae GN6 as experimental strain, the effects of phytohormones such as seaweed liquid fertilizer, sodium nitrophenolate, and indol-3-acetic acid and vitamins such as vitamin B1 and vitamin B12on the growth and oil production of microalgae GN6 were researched. Based on single factor experiment, four factors and three levels orthogonal experiment was used to optimize the culture conditions. The results showed that with BG11 as the basic culture medium, the optimal culture conditions of microalgae GN6 were obtained as follows:1.5 mL/L seaweed liquid fertilizer, 15 關g/L vitamin B12, 0.60 g/L peptone and 20 g/L glucose. Under the optimal conditions, the biomass and oil productivity were 16.4 g/L and 1.62 g/L, respectively, which were 100.3% and 52.2% higher than those before optimization. Therefore, these results could provide a beneficial exploration for the further improvement of growth and oil production of oil-rich microalgae by adding phytohormones and vitamins. |
