Effects of melatonin on accumulation of astaxanthin and synthesis of fatty acid in H.pluvialis LUGU
  
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KeyWord:melatonin  H.pluvialis LUGU  astaxanthin  fatty acid  enzyme activity
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YUE Chenchen,DING Wei, ZHAO Peng,etc  
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Abstract:
      The effects of melatonin (MLT)on the algae cell growth, astaxanthin accumulation, fatty acids, and fatty acid synthesis-related enzyme activities of H. pluvialis LUGU were studied. The results showed that the astaxanthin content in algae cells achieved 26.62 mg/g under the induction conditions of MLT concentration 10 關mol/L and cultivation time 13 d, which was 2.16 times as high as that of the control (12.3 mg/g). The content of fatty acids (15.32%) was the highest when cultivating for 5 d, which was 1.24 times as high as that of the control, and the astaxanthin ester content increased. Moreover, the acetyl-CoA carboxylase (ACCase) activity significantly increased, but the phosphoenolpyruvate carboxylase (PEPCase) activity reduced by MLT induction.The study revealed that the appropriate concentration of MLT not only could improve the astaxanthin accumulation in H. pluvialis LUGU, but also could boost the fatty acid content and change the fatty acid composition.
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