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Determination of content of nervonic acid separated from Malania oleifera oil by HPLC
  
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KeyWord:high performance liquid chromatography  nervonic acid  Malania oleifera oil
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LAI Fubing，LI Weiguang，LAI Fang，etc  
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Abstract:
      A method for determination of content of nervonic acid separated from Malania oleifera oil was established. The method was developed under the following conditions: Hypersil ODS2 (4.6 mm×250 mm, 5 μm), mobile phase of acetonitrile-methanol-0.4% acetic acid solution-tetrahydrofuran (volume ratio 80∶[KG-*2]12∶[KG-*2]5∶[KG-*2]3), flow rate 1.0 mL/min, injection volume 5 μL, column temperature 26[KG-*8]℃ and detection wave length 205 nm. The results showed that the standard curve of nervonic acid had good linear in the range of 1.0-20.0 mg/mL. The relative standard deviation (RSD) of precision and stability were 1.52% and 1.61%, respectively. The recovery rate of nervonic acid was 97.41%-104.89%, and RSD was 0.72%-3.22%. The RSD of the tested sample was 0.90%-2.36%. The method was simple, rapid, accurate and reproducible, and it could be used to determine the content of nervonic acid during the separation of fatty acids from Malania oleifera oil.
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