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| Determination of cholesterol content in edible oils and fats by gel permeation chromatography combined with high performance liquid chromatography-evaporative light scattering detector |
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| KeyWord:edible oils and fats cholesterol gel permeation evaporative light scattering detector |
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| Abstract: |
| A method for the determination of cholesterol content in edible oils and fats was established by gel permeation chromatography system combined with liquid chromatography-evaporative light scattering detector. The results showed that the linearity correlation was good in the range of 2-200 μg/mL with correlation efficient 0.998 9. The limit of detection, recovery rate and relative standard deviation were 10 mg/kg, 94.01%-103.80% and 1.12%-7.25% respectively. The method could effectively reduce the loss of cholesterol caused by cumbersome pre-treatment process, and avoid solvent response and solvent peak interference during detection. The method was proved to be simple, feasible and effective. |
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