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| Hydrolysis of olive oil by lipase in three liquid phase system |
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| KeyWord:three liquid phase oil hydrolysis olive oil lipase |
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| Abstract: |
| The effects of the materials on the enzyme in three liquid phase system were investigated by hydrolyzing olive oil in three liquid phase catalytic system. The contents of ammonium sulfate and PEG400 on the partition coefficient, recovery and catalytic efficiency of the lipase were investigated. The effect of the volume ratio of middle-bottom phase to oil on the hydrolysis of olive oil and the microdroplet structure of emulsion in three liquid phase system were studied. The results showed that under the conditions of enzyme dosage 142.5 U/g, ammonium sulfate content 15%, PEG400 content 28%, volume ratio of middle-bottom phase to oil 3∶?1, and agitation speed 200 r/min, the partition coefficient and recovery of the lipase were 28.28 and 99.65%, respectively. And the fatty acid content was 98.17% after hydrolysis in three liquid phase system for 24 h, while in the oil-water two phase system, the fatty acid content was only 42.06%. The unique droplet structure in the three liquid phase system might be the reason that the system was superior to the oil-water two phase system. |
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