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| Synthesis of 1,3-distearoyl-2-oleoylglycerol by enzymatic acidolysis in solvent-free system |
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| DOI： |
| KeyWord:1, 3-distearoyl-2-oleoylglycerol acidolysis NS40086 lipase |
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| Abstract: |
| Products rich in 1,3-distearoyl-2-oleoylglycerol (SOS) was synthesized by enzymatic acidolysis of high-oleic sunflower seed oil and stearic acid catalyzed by immobilized 1,3-regiospecific lipase in solvent-free system,and the synthesis process was optimized. The effects of lipase type, molar ratio of high-oleic sunflower seed oil to stearic acid, reaction time and reaction temperature on the content of SOS were investigated. The results showed that the optimal conditions of synthesis of SOS in solvent-free system were obtained as follows: with immobilized lipase NS40086 as catalyst and its dosage 12%(based on total mass of substrates), molar ratio of high-oleic sunflower seed oil to stearic acid 1∶?12, reaction time 4 h and reaction temperature 70?℃. Under these conditions, the content of SOS,SSO and SOO in the product were 661%，7.1% and 13.5%, respectively. |
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