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| 桃仁多肽的分离及其抗氧化性能研究 |
| Isolation of peach kernel polypeptide and its antioxidant capacities |
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| DOI： |
| 中文关键词: 桃仁 多肽 抗氧化性能 超滤 相对分子质量 |
| 英文关键词:peach kernel polypeptide antioxidant capacity ultrafiltration relative molecular weight |
| 基金项目: |
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| 中文摘要: |
| 桃仁是一种优质的油料和蛋白质资源，为获得具有高抗氧化活性的桃仁多肽，通过超滤技术对酶解法制备的桃仁多肽（PSP）进行分级分离，得到3种相对分子质量分布不同的组分PSP-1、PSP-2和PSP-3，并以4种不同的抗氧化能力实验为指标进行评定。结果表明：相对分子质量分布不同的桃仁多肽其抗氧化能力差异明显，其中相对分子质量低于1 kDa的PSP-1具有最高的DPPH·清除能力（IC50=1.44 mg/mL）、OH·清除能力（IC50=1.04 mg/mL）、O-2·清除能力和还原能力。因此，采用截留相对分子质量为1 kDa的超滤膜可有效分离得到具有高抗氧化活性的桃仁多肽。 |
| 英文摘要: |
| Peach kernel is a high-quality resource of oil and protein. To obtain polypeptide with high antioxidant activities from peach kernel，ultrafiltration was used to isolate peach kernel polypeptide (PSP) prepared by enzymolysis method. And three constituents (PSP-1, PSP-2, PSP-3) with different relative molecular weight distribution were separated and collected. Then the antioxidant capacities of these constituents were investigated through four evaluation systems. The results showed that antioxidant capacities of peach kernel polypeptide with different relative molecular weights had significant differences. PSP-1 with relative molecular weight below 1 kDa showed the highest DPPH radical scavenging activity [JP2](IC50=1.44 mg/mL), hydroxyl radical scavenging activity (IC50=1.04 mg/mL), superoxide anion radical scavenging activity and reducing power. So ultrafiltration membrane with retention relative molecular weight 1 kDa could be applied to the isolation of peach kernel polypeptide with high antioxidant capacity. |
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