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| Insufficient supplement of glucose enforcing a large accumulation of carotenoids by Schizochytrium sp.S31 |
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| KeyWord:Schizochytrium sp.S31 carotenoids carbon source lipid |
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| Abstract: |
| For exploring the nutrient regulation to enforce accumulation of carotenoids by Schizochytrium sp. S31, the effects of different amounts of glucose and yeast extract on the biomass, synthesis of lipids and carotenoids in the middle stage of fermentation (beginning at 72 h) were investigated. The results indicated that the carotenoids content in cell was relatively higher when glucose was fed at a lower level, although the biomass and lipids production were low. Furthermore, the fermentations were carried out in a 5 L fermentor, using DO-Stat glucose feeding strategy in the middle stage, in order to create the condition of insufficient glucose supplement on purpose. The highest carotenoids content 151.0 μg/g was achieved when the fermentation time was 98 h, more than four times increment compared with the level of fermentation with glucose sufficient supplement. From analysis of the fermentation performances and metabolic pathway, it could be speculated that insufficient glucose supplement in the lipid synthesis stage could enforce carbon source flowing into the carotenoids synthesis pathway, by restricting cell growth and weakening the lipid in cell synthesis pathway, which improved a large accumulation of carotenoids. |
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