Effects of fulvic acid on lipid accumulation and genes (me, pepc, accD ) expression of Monoraphidium sp. FXY-10
  
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KeyWord:Monoraphidium sp. FXY-10  fulvic acid  lipid  ROS  gene expression
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DONG Xunzan, CHE Raoqiong, ZHAO Yongteng, etc  
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Abstract:
      The effects of fulvic acid on the biomass, lipid content, reactive oxygen(ROS) level and the expression of lipid biosynthesis-related genes of Monoraphidium sp. FXY-10 were studied. Two stage culture method(first heterotrophic and then photoautotrophic) was used and fulvic acid was added to induce. The results showed that the lipid content reached 54.3% in algal cells treated with 25 mg/L fulvic acid, which was 1.12 times of the control, and the intracellular ROS level was 1.46 times of the control. Moreover, the lipid biosynthesis-related genes was correlated with the lipid synthesis, and the expressions of me, accD and pepc were 1.32 times(4 d), 1.67 times(4 d) and 1.71 times(6 d) of the control. These results demonstrated that fulvic acid significantly increased microalgae lipid accumulation by changing the ROS level and gene expression.
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