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| Effect of carbon and nitrogen mass concentrations on lipid production and transcriptional regulation of SNF1 complex in Mortierella alpina |
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| KeyWord:Mortierella alpina SNF1 complex lipid accumulation nutrition signal transcriptional regulation |
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| Abstract: |
| Mortierella alpina is an oleaginous filamentous fungus with strong oil producing capacity, and its lipid synthesis is regulated by carbon and nitrogen in culture medium. The effects of different mass concentrations of glucose and ammonium tartrate on biomass and lipid accumulation of M. alpina were studied. Under different mass concentrations of carbon and nitrogen,the transcriptional levels of subunits of M. alpina SNF1 complex were further studied.The results showed that lipid accumulation was basically proportional to carbon to nitrogen ratio(C/N) under the same initial nitrogen mass concentration. When C/N was 24.6, the fatty acid content was 42.6%, higher than that of C/N 4.6. While under the same initial glucose mass concentration, in nitrogen limited culture medium(C/N 76.7), fatty acid yield reached 2.6 g/L, which was 1.5-3.2 times of that in nitrogen-rich medium, indicating that nitrogen mass concentration showed a greater impact on lipid production. High glucose mass concentration(C/N 24.6) led to 13.9-20.5 times higher transcriptional level of SNF1 complex compared with lower glucose groups under the same nitrogen level, and higher C/N brought by nitrogen limitation also promoted SNF1 transcription. High C/N and nitrogen limitation were both unbalanced nutrition signal, and changes in transcriptional level might be the response of M. alpina SNF1 complex to nutrient signal, which had positive relationship to lipid accumulation. |
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