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| Enrichment of nervonic acid from Xanthoceras sorbifolia Bunge. oil by urea inclusion fractionation |
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| DOI： |
| KeyWord:Xanthoceras sorbifolia Bunge. oil nervonic acid urea inclusion fractionation enrichment technology gas chromatography(GC) |
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| Abstract: |
| Using Xanthoceras sorbifolia Bunge. seed as raw material, the Xanthoceras sorbifolia Bunge. oil was produced by low-temperature pressing. The fatty acid composition of Xanthoceras sorbifolia Bunge. oil was detected and the nervonic acid in Xanthoceras sorbifolia Bunge. oil was enriched by urea inclusion fractionation. With content and recovery of nervonic acid as the indexes, the enrichment conditions were optimized by single factor experiment and orthogonal experiment. The results showed that there were eight kinds of fatty acids with content above 1% in Xanthoceras sorbifolia Bunge. oil. The primary fatty acids were oleic acid (27.76%) and linoleic acid (44.87%), and the rare nervonic acid content was 2.59%. The optimal enrichment conditions were determined as follows: ratio of urea to fatty acids 1∶?1, ratio of material to liquid 1∶?10, inclusion temperature 10?℃ and inclusion time 8 h. Under the optimal conditions, the recovery of nervonic acid was 74.01% and the content of nervonic acid reached 949%, which increased about three times than that before inclusion and about one time higher than that in the rare Acer truncatum seed oil (about 5%) , which was a typical functional oil. |
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